Iindaba - Uthini umahluko phakathi kwe-pH electrode enye kunye nephindwe kabini?

Ii-electrode ze-PH ziyahluka ngeendlela ezininzi; ukusuka kwimilo yencam, isiphambuka, imathiriyeli kunye nokuzaliswa. Umahluko ophambili kukuba ingaba i-electrode ine-junction eyodwa okanye kabini.

Zisebenza njani ii-electrode ze-pH?
Ii-electrode ze-pH ezidityanisiweyo zisebenza ngokuba ne-sensing half-cell (i-AgCl egqunywe ngocingo lwesilivere) kunye ne-reference half-cell (Ag/AgCl reference electrode wire), la malungu mabini kufuneka adityaniswe kunye ukuze agqibezele isekethe ukuze imitha ifumane i-pH yokufunda. Ngelixa isiqingatha seseli semvakalelo siva utshintsho kwi-pH yesisombululo, isiqingatha seseli yereferensi siseno esizinzileyo. I-Electrodes ingaba lulwelo okanye ijeli izaliswe. I-electrode ye-junction ye-liquid idala i-junction kunye nefilimu encinci yokuzalisa isisombululo kwi-tip ye-probe. Ngokuqhelekileyo banomsebenzi wempompo ukuvumela ukuba wenze i-junction entsha yokusetyenziswa ngakunye. Bafuna ukugcwaliswa kwakhona rhoqo kodwa banikezela ngeyona ndlela isebenzayo eyandisa ubomi babo bonke, ukuchaneka kunye nesantya sokuphendula. Ukuba sigcinwe isiphambuka solwelo siya kuba nobomi obungunaphakade obusebenzayo. Ezinye ii-electrode zisebenzisa ijeli ye-electrolyte ekungeyomfuneko ukuba igqitywe ngumsebenzisi. Oku kubenza babe lolona khetho lwasimahla ngakumbi kodwa luyakunciphisa ubomi be-electrode ukuya malunga nonyaka om-1 ukuba igcinwe ngokuchanekileyo.

I-Junction ephindwe kabini - ezi electrode ze-pH zinebhulorho yetyuwa eyongezelelweyo ukukhusela ukuphendula phakathi kwesisombululo sokuzalisa i-electrode kunye nesampuli yakho enokuthi ibangele umonakalo kwi-electrode junction. Kufuneka bavavanye iisampulu eziqulethe iiproteni, isinyithi esinzima okanye i-sulphides

ISingxulo esiNye – ezi zezenjongo jikelele yezicelo zeesampulu ezingayi kuvala isiphambuka.

Loluphi uhlobo lwe-pH electrode ekufuneka ndilusebenzisa?
Ukuba isampulu ineeprotheyini, iisulphites, isinyithi esinzima okanye i-TRIS buffers i-electrolyte inokusabela kunye nesampulu kwaye yenze i-precipitate eqinileyo evala ukudibana kwe-porous ye-electrode kwaye iyimise ukusebenza. Le yenye yezona zizathu zixhaphakileyo ze "electrode efileyo" esiyibonayo amaxesha ngamaxesha.

Kwezo sampuli ufuna ukuhlangana kabini - oku kubonelela ngokhuseleko olongezelelweyo kule nto yenzekayo, ke uya kufumana ubomi obungcono kakhulu ngaphandle kwe-pH electrode.