Kwi-bioprocessing, ukugcina ulawulo oluchanekileyo lweemeko zokusingqongileyo kubalulekile.Eyona nto ibalulekileyo kwezi meko yi-pH, echaphazela ukukhula kunye nemveliso ye-microorganisms okanye iiseli ezisetyenziswa kwizicelo ezahlukeneyo ze-biotechnological.Ukufezekisa olu lawulo luchanekileyo, abaqhubi be-bioreactor baxhomekeke kwizixhobo eziphambili kunye nezinzwa-eyona nto ibalulekileyo kukubabioreactor inzwa pH.
I-Bioreactor pH Sensor: Imigaqo eSisiseko yoMlinganiselo we-pH
1. I-Bioreactor pH Sensor: Inkcazo ye-pH
I-pH, okanye "ithuba le-hydrogen," ngumlinganiselo we-asidi okanye i-alkalinity yesisombululo.Ilinganisa i-concentration ye-hydrogen ions (H +) kwisisombululo esinikiweyo kwaye ibonakaliswe kwisikali se-logarithmic ukusuka kwi-0 ukuya kwi-14, kunye ne-7 emele ukungathathi hlangothi, ixabiso elingaphantsi kwe-7 elibonisa i-acidity, kunye nexabiso elingaphezulu kwe-7 ebonisa i-alkalinity.Kwi-bioprocessing, ukugcinwa kwenqanaba elithile le-pH kubalulekile ekukhuleni okugqibeleleyo kunye nemveliso ye-microorganisms okanye iiseli.
2. I-Bioreactor pH Sensor: i-pH Scale
Ukuqonda isikali se-pH kubalulekile ekuqondeni ukubaluleka kokubeka iliso kwi-pH.Ubume belogarithmic yesikali buthetha ukuba utshintsho lweyunithi enye lubonisa umahluko ophindwe kashumi kugxininiso lwe-hydrogen ion.Obu buntununtunu benza ulawulo lwe-pH oluchanekileyo lubaluleke kakhulu kwii-bioreactors, apho ukutenxa okuncinci kunokuchaphazela kakhulu inkqubo yebhayoloji.
3. I-Bioreactor pH Sensor: Ukubaluleka kokuHlolwa kwe-pH kwi-Bioprocessing
I-Bioprocessing ibandakanya izicelo ezahlukeneyo, kubandakanya ukuvutshelwa, imveliso ye-biopharmaceutical, kunye nokucocwa kwamanzi amdaka.Kwinkqubo nganye kwezi, ukugcina uluhlu oluthile lwe-pH lubalulekile ekulawuleni ukusabela kwe-enzymatic, ukukhula kwe-microbial, kunye nomgangatho wemveliso.Ukujongwa kwe-pH kuqinisekisa ukuba imeko-bume ye-bioreactor ihlala ngaphakathi kwemilinganiselo efunekayo, ukwandisa imveliso kunye nemveliso yemveliso.
4. I-Bioreactor pH Sensor: Izinto ezichaphazela i-pH kwii-Bioreactors
Izinto ezininzi zinokuchaphazela amanqanaba e-pH ngaphakathi kwe-bioreactors.Ezi ziquka ukongezwa kwezinto ezinobumuncu okanye ze-alkali, i-metabolic byproducts ye-microorganisms, kunye nokutshintsha kweqondo lokushisa.Ukubeka iliso kunye nokulawula ezi ziguquguqukayo ngexesha lokwenyani lwenziwa ukuba lwenzeke zizinzwa ze-pH, ezidlala indima ebalulekileyo kulawulo lwe-bioprocess.
I-Bioreactor pH Sensor: Iintlobo ze-pH Sensors
1. I-Bioreactor pH Sensor: I-Glass Electrode pH Sensors
I-Glass electrode sensors ye-pH yenye yezona ntlobo ziqhelekileyo ezisetyenziswa kwi-bioprocessing.Ziquka inwebu yeglasi ephendula utshintsho kwi-ion concentration ye-hydrogen.Ezi zivamvo zaziwa ngokuchaneka kwazo kunye nokuthembeka, nto leyo ezenza zibe lukhetho olukhethwayo kwizicelo ezibalulekileyo ze-bioreactor.
2. I-Bioreactor pH Sensor: ISFET (Ion-Selective Field-Effect Transistor) iiSensori ze-pH
Izinzwa ze-ISFET ze-pH zizixhobo zemeko eyomeleleyo ezibona utshintsho lwe-pH ngokulinganisa amandla ombane kwitshiphu yesilicon.Banikezela ngezinto eziluncedo ezinjengokuqina kunye nokufaneleka kokusetyenziswa kosetyenziso olunye, okubenza ukuba babe kukhetho oluguquguqukayo kwi-bioprocessing.
3. I-Bioreactor pH Sensor: Reference Electrodes
Ii-electrodes ezibhekiselele yinxalenye ebalulekileyo yeenzwa ze-pH.Babonelela ngereferensi ezinzileyo apho i-electrode yeglasi ilinganisa i-pH.Ukhetho lwereferensi ye-electrode inokuchaphazela ukusebenza kwenzwa, kwaye ukukhetha indibaniselwano eyiyo kubalulekile kumlinganiselo ochanekileyo we-pH.
4. I-Bioreactor pH Sensor: Ukuthelekiswa kweeNdidi zeSensor
Ukukhetha i-sensor ye-pH efanelekileyo kwisicelo se-bioprocessing kuxhomekeke kwizinto ezifana nokuchaneka, ukuqina, kunye nokuhambelana neemfuno zenkqubo ethile.Ukuthelekiswa kweentlobo ezahlukeneyo zenzwa kuya kunceda iingcali ze-bioprocess ukuba zenze izigqibo ezinolwazi xa zikhetha izixhobo zokubeka iliso ze-pH.
I-Bioreactor pH Sensor: i-Bioreactor pH Sensor Design
1. I-Bioreactor pH Sensor: iSensor Housing
I-sensor yendlu yigobolondo yangaphandle ekhusela amacandelo angaphakathi ukusuka kwindawo enzima ngaphakathi kwe-bioreactor.Xa ukhetha izinto zendlu, kubalulekile ukuqwalasela izinto ezinjengokuhambelana kweekhemikhali, ukuqina, kunye nokulula kokucoca.Insimbi engenasici yinto esetyenziswa ngokuqhelekileyo ngenxa yokuchasana nokubola kunye nokuqina.Ubume kunye nobukhulu bendlu kufuneka iyilwe ukuba ilingane iimfuno ezithile ze-bioreactor ngelixa kuqinisekiswa lula ukufakela kunye nokugcinwa.
2. I-Bioreactor pH Sensor: iSensing Element
Intliziyo yenzwa ye-pH yinto yayo yokuva.Iinzwa ze-Bioreactor pHngokwesiqhelo basebenzise i-electrode yeglasi okanye i-Ion-Sensitive Field Effect Transistor (ISFET) njengesixhobo sokuva.Iielectrode zeglasi ziyaziwa ngokuchaneka kwazo kunye nokuthembeka, ngelixa ii-ISFET zibonelela ngeenzuzo ngokubhekiselele kwi-miniaturization kunye nokomelela.Ukhetho phakathi kwezi zimbini ubukhulu becala luxhomekeke kwiimfuno zesicelo.Ukukhetha isisombululo esifanelekileyo se-electrolyte ngaphakathi kwendawo yokuva kubalulekile ukugcina ukusebenza kwe-electrode ngokuhamba kwexesha.
3. I-Bioreactor pH Sensor: Reference Electrode
I-electrode yereferensi iyimfuneko kumlinganiselo we-pH njengoko ibonelela ngenqaku elizinzileyo lokubhekisela.Kukho iindidi ngeendidi zeereferensi zereferensi, kuquka iAg/AgCl kunye neCalomel electrode.Ukuqwalaselwa kwesondlo kubandakanya ukugcina i-reference electrode junction icocekile kwaye uqinisekise ukuba isisombululo sereferensi sihlala sizinzile.Ukujonga rhoqo kunye nokuzalisa isisombululo sereferensi kuyimfuneko ukugcina ukuchaneka.
4. I-Bioreactor pH Sensor: I-Junction Design
Uyilo lwe-junction yenzwa ye-pH ibalulekile ekugcineni ukuhamba kweeyoni phakathi kwesisombululo senkqubo kunye ne-electrode yereferensi.Olu luyilo kufuneka luthintele ukuvaleka kunye nokunciphisa ukukhukuliseka ekufundeni.Ukukhethwa kwezinto ezidibeneyo kunye nokucwangciswa kwayo kudlala indima ebalulekileyo ekusebenzeni jikelele kwenzwa.
6. I-Bioreactor pH Sensor: Iinkqubo zokuCalibration
Ulungelelwaniso linyathelo elibalulekileyo ekuqinisekiseni imilinganiselo ye-pH echanekileyo.Izibonisi ze-pH kufuneka zilinganiswe rhoqo kusetyenziswa izisombululo eziqhelekileyo zesithinteli ezinamaxabiso aziwayo e-pH.Iinkqubo zokulinganisa kufuneka zilandelwe ngenyameko, kwaye iirekhodi zokulinganisa kufuneka zigcinwe ukulungiselela iinjongo zokulandelela kunye nokulawula umgangatho.
I-Bioreactor pH Sensor: Ukufakela kunye nokudibanisa
1. I-Bioreactor pH Sensor: Ukubekwa ngaphakathi kwe-Bioreactor
Ukubekwa ngokufanelekileyo kweenzwa ze-pH ngaphakathi kwe-bioreactor kubalulekile ukufumana imilinganiselo yommeli.Izinzwa kufuneka zibekwe ngobuchule ukuze zibeke iliso kwiiyantlukwano ze-pH kuyo yonke inqanawa.Ufakelo kufuneka kwakhona luqwalasele izinto ezifana nokuqhelaniswa nenzwa kunye nomgama ukusuka kwi-agitator.
2. I-Bioreactor pH Sensor: Uqhagamshelwano kwiiNkqubo zoLawulo
I-Bioreactor pH sensors kufuneka idibaniswe kwinkqubo yokulawula ye-bioreactor.Oku kubandakanya ukudibanisa inzwa kwi-transmitter okanye isilawuli esinokutolika ukufundwa kwe-pH kunye nokwenza uhlengahlengiso oluyimfuneko ukuze kugcinwe inqanaba le-pH elifunekayo.
3. I-Bioreactor pH Sensor: Iingqwalasela zeCable kunye neConnector
Ukukhetha iintambo ezifanelekileyo kunye nezihlanganisi zibalulekile kuthumelo lwedatha oluthembekileyo kunye nobomi obude.Iintambo kufuneka ziyilelwe ukumelana neemeko ezinzima ngaphakathi kwe-bioreactor, kwaye izihlanganisi kufuneka zinganyangeki kwi-corrosion ukuze kugcinwe uxhumano lombane oluzinzileyo.
I-Bioreactor pH Sensor: Ulungelelwaniso kunye noLondolozo
1. I-Bioreactor pH Sensor: Iinkqubo zokuCalibration
Ukulinganisa rhoqo kubalulekile ukuqinisekisa imilinganiselo ye-pH echanekileyo.Ukuphindaphinda kokulinganisa kuxhomekeke kwizinto ezifana nokuzinza kwenzwa kunye nokubaluleka kolawulo lwe-pH kwinkqubo.Kucetyiswa ukuba ulandele izikhokelo zomenzi kwiinkqubo zokulinganisa.
2. I-Bioreactor pH Sensor: Frequency of Calibration
Ukuphindaphinda kokulinganisa kufuneka kumiselwe ngokusekelwe kwisicelo esithile kunye nokuzinza kwenzwa.Ezinye izixhobo zoluvo zinokufuna ulungelelwaniso oluthe kratya, ngelixa ezinye zinokugcina ukuchaneka ixesha elide.
3. I-Bioreactor pH Sensor: Ukucoca kunye noLondolozo
Ukucoca nokugcinwa ngokufanelekileyo kubalulekile kwi-sensor ixesha elide kunye nokuchaneka.Iinkqubo zokucoca kufuneka zenziwe rhoqo ukususa nayiphi na i-biofilm okanye idiphozithi enokuthi iqokelele kwi-sensor surface.Ukugcinwa kufuneka kubandakanye ukujonga i-electrode yereferensi kunye ne-junction yeempawu zokugqoka okanye ukungcola.
4. I-Bioreactor pH Sensor: Ukuxazulula iingxaki kwiMiba eqhelekileyo
Ngaphandle koyilo olululo kunye nokugcinwa ngokufanelekileyo, izinzwa ze-pH zinokuhlangabezana nemiba enje nge-drift, ingxolo yesignali, okanye i-electrode fouling.Iinkqubo zokusombulula ingxaki kufuneka zibekho ukuchonga nokusombulula le miba ngokukhawuleza ukunciphisa ukuphazamiseka kwenkqubo.
Ukuqukumbela
Ibioreactor inzwa pHsisixhobo esibalulekileyo kwi-bioprocessing, evumela ulawulo oluchanekileyo lwamanqanaba e-pH ukwandisa ukukhula kwe-microbial kunye nemveliso yemveliso.Ukuqonda imigaqo esisiseko yomlinganiselo we-pH kunye neendidi ezahlukeneyo zezivamvo ze-pH ezikhoyo kuxhobisa abaqhubi be-bioprocess ukuba benze ukhetho olunolwazi ekukhetheni ezona zixhobo zifanelekileyo kwizicelo zabo.Ngeenzwa ezithembekileyo ze-pH ezivela kubaboneleli abafana ne-Shanghai BOQU Instrument Co., Ltd., iingcali ze-bioprocessing zinokuqhubeka nokuqhubela phambili intsimi ye-biotechnology kwaye zivelise iimveliso ezikumgangatho ophezulu ngokufanelekileyo.
Ixesha lokuposa: Sep-13-2023
